Development and characterization of genic-SSR markers from different Asia lotus (Nelumbo nucifera) types by RNA-seq
نویسندگان
چکیده
منابع مشابه
Characterization of Flower-Bud Transcriptome and Development of Genic SSR Markers in Asian Lotus (Nelumbo nucifera Gaertn.)
BACKGROUND Asian lotus (Nelumbo nucifera Gaertn.) is the national flower of India, Vietnam, and one of the top ten traditional Chinese flowers. Although lotus is highly valued for its ornamental, economic and cultural uses, genomic information, particularly the expressed sequence based (genic) markers is limited. High-throughput transcriptome sequencing provides large amounts of transcriptome d...
متن کاملRNA-Seq Uncovers SNPs and Alternative Splicing Events in Asian Lotus (Nelumbo nucifera)
RNA-Seq is an efficient way to comprehensively identify single nucleotide polymorphisms (SNPs) and alternative splicing (AS) events from the expressed genes. In this study, we conducted transcriptome sequencing of four Asian lotus (Nelumbo nucifera) cultivars using Illumina HiSeq2000 platform to identify SNPs and AS events in lotus. A total of 505 million pair-end RNA-Seq reads were generated f...
متن کاملEfficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues.
Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-quality RNA applicable for evaluation ...
متن کاملCloning and characterization of the UBC gene from lotus (Nelumbo nucifera).
Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNA NnUBCs of Nelumbo nucifera. With the same coding sequence le...
متن کاملEthno-medicinal uses and pharmacological activities of lotus (Nelumbo nucifera)
Nelumbo nucifera is grown in many parts of the globe including India for its medicinal and nutritional value. In Kashmir, the plant grows naturally in the lakes and its stem is being extensively used in many famous Kashmiri cuisines. In addition, its fruits and seeds are also consumed, but to a lesser extent. Many studies have established a wide range of the pharmacological activities of this p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2015
ISSN: 1676-5680
DOI: 10.4238/2015.september.22.11